Yeast surface display for protein engineering and characterization
نویسندگان
چکیده
منابع مشابه
Engineering antibody affinity by yeast surface display.
Yeast surface display (YSD) is a powerful tool for engineering the affinity, specificity, and stability of antibodies, as well as other proteins. Since first described in 1997 by Boder and Wittrup, YSD has been employed successfully in engineering a number of antibodies, as well as T-cell receptors. A recently reported large nonimmune single chain antibody library serves as a good starting poin...
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overview of systems and techniques for surface display of recombinant proteins in yeast s. cerevisiae
in the past decade much effort has been devoted to the development of new expression systems and novel techniques for the surface display of heterologous proteins in yeast in order to improve their applications in biotechnology, food technology, pharmacology and medicine. heterologous protein-encoding genes are generally fused with genes coding for yeast cell wall proteins or their fragments re...
متن کاملBeyond antibody engineering: directed evolution of alternative binding scaffolds and enzymes using yeast surface display.
Pioneered exactly 20 years ago, yeast surface display (YSD) continues to take a major role in protein engineering among the high-throughput display methodologies that have been developed to date. The classical yeast display technology relies on tethering an engineered protein to the cell wall by genetic fusion to one subunit of a dimeric yeast-mating agglutination receptor complex. This method ...
متن کاملYeast surface display for directed evolution of protein expression, affinity, and stability.
The described protocols enable thorough screening of polypeptide libraries with high confidence in the isolation of improved clones. It should be emphasized that the protocols have been fashioned for thoroughness, rather than speed. With library plasmid DNA in hand, the time to plated candidate yeast display mutants is typically 2-3 weeks. Each of the experimental approaches required for this m...
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ژورنال
عنوان ژورنال: Current Opinion in Structural Biology
سال: 2007
ISSN: 0959-440X
DOI: 10.1016/j.sbi.2007.08.012